| Autor: |
Hondmann DH; Department of Genetics, Agricultural University, HA Wageningen, The Netherlands., Visser J |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1990 Jun 27; Vol. 510, pp. 155-64. |
| DOI: |
10.1016/s0021-9673(01)93749-5 |
| Abstrakt: |
A method is described by means of which 96 different dye-adsorbents can be tested simultaneously for their ability to bind enzymes and to test their biospecific elution. Small amounts of cell-free extract are applied to dye-adsorbents which are packed in a 96-well transplate cartridge. After biospecific elution, the amount of the eluted enzyme is tested in a microtitre plate assay. The method is illustrated by the purification of glycerol dehydrogenase (E.C. 1.1.1.72), 6-phosphogluconate dehydrogenase (E.C. 1.1.1.44) and glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49) from the hyphal fungus Aspergillus nidulans. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
