Improving the sensitivity of immunoassays with PEG-COOH-like film prepared by plasma-based technique.

Autor: O'Mahony CC; Biomedical Diagnostics Institute, Dublin City University, Dublin 9, Ireland., Gubala V, Gandhiraman RP, Daniels S, Yuk JS, Maccraith BD, Williams DE
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2012 Jan; Vol. 100 (1), pp. 230-5. Date of Electronic Publication: 2011 Oct 31.
DOI: 10.1002/jbm.a.33268
Abstrakt: Herein we report on a preparation and performance of stable, hydrophilic and biocompatible polymeric material suitable for functionalization of disposable substrates used in biosensors. This new material features COOH surface groups cross-linked with ethylene glycol molecules and was prepared in situ on disposable, plastic substrate by high-throughput and environmentally friendly technique called plasma-enhanced chemical vapor deposition (PECVD). The film is grafted to the plasma activated plastic by sequential deposition of tetraethylorthosilicate, forming a bonding layer, and mixed vapors of acrylic acid and diethyleneglycol dimethylether (AA/PEG) that provide the desired functional groups forming a sensing, contact layer. A superior performance of the AA/PEG coating as suitable material for substrates in biomedical devices was demonstrated in a model fluorescence linked immunosorbent assay. The results were compared with other commonly used surface materials prepared by wet chemistry methods. The unique characteristic of the AA/PEG film is that the immunoassay can be executed without the need for a blocking step, typically using albumins, without negative consequences on the bioassay results. In fact, the superior quality of the materials modified with AA/PEG film was highlighted by improving the sensitivity of an immunoassay by two orders of magnitude when compared with substrates prepared by standard surface chemistry methods.
(Copyright © 2011 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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