| Autor: |
Zhou AJ; Oral & Maxillofacial Surgery, Faculty of Dentistry, University of Toronto, 124 Edward Street, Room 412, Toronto, Toronto, ON, M5G 1G6, Canada., Clokie CM, Peel SA |
| Jazyk: |
angličtina |
| Zdroj: |
Biotechnology letters [Biotechnol Lett] 2012 Feb; Vol. 34 (2), pp. 221-30. Date of Electronic Publication: 2011 Oct 22. |
| DOI: |
10.1007/s10529-011-0781-7 |
| Abstrakt: |
To improve recombinant human bone morphogenetic protein-2 (rhBMP-2) yield, cell lines stably expressing hBMP2 were cultured in the presence of polyarginine peptide IND-1 and showed up to 6-fold increase in the yield of mature BMP-2. Repeated addition of IND-1 to cell cultures consistently improved BMP-2 yields over 53 days without affecting cell growth and viability. Investigation of its mechanism of action showed that IND-1 inhibited pro-protein convertase (PC) activity when incubated with cell lysates. However, when intact cells were cultured with IND-1, no change in cellular PC activity was observed. Furthermore, knockdown of furin (a prototypical member of the PCs) in cells did not affect their BMP-2 yields, suggesting furin/PC inhibition is unlikely the mechanism by which IND-1 enhances BMP-2 yields. IND-1 as a medium additive thus enhances BMP-2 production in mammalian cell expression systems. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink