| Autor: |
Oprandy JJ; Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20814., Long GW |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of clinical microbiology [J Clin Microbiol] 1990 Aug; Vol. 28 (8), pp. 1701-3. |
| DOI: |
10.1128/jcm.28.8.1701-1703.1990 |
| Abstrakt: |
Data on a technique for the detection of antigen from arthropod vectors in a dot immunobinding assay are presented. In this system, antigen present in the vector was first solubilized in sodium dodecyl sulfate. The homogenate from this process was microfiltered through a two-membrane sandwich; target antigen molecules passed through the first membrane and were immobilized on the second one. The first membrane was nonbinding and served to impinge debris. The second membrane was a high-protein-binding-capacity hydrophobic polyvinylidene difluoride membrane. High signal-to-noise ratios were produced by this method, which is readily adaptable for field use. This assay was used for malaria sporozoites, but it can serve as a general technique that is applicable to other arthropod vectors and etiologic agents. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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