Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes.

Autor: Takahashi N; Department of Internal Medicine, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023, Japan. ntkhs@hoku-iryo-u.ac.jp, Yoshizaki T, Hiranaka N, Suzuki T, Yui T, Akanuma M, Oka K, Kanazawa K, Yoshida M, Naito S, Fujiya M, Kohgo Y, Ieko M
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2011 Nov 11; Vol. 415 (1), pp. 200-5. Date of Electronic Publication: 2011 Oct 18.
DOI: 10.1016/j.bbrc.2011.10.060
Abstrakt: Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome. However, the significance of this reduction remains unclear. This study investigated if and how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and protein expression levels of monocyte chemoattractant protein-1 (MCP-1) were significantly increased, although the other genes tested were not altered. The conditioned media from the cells promoted monocyte chemotaxis. The increase in MCP-1 expression was prevented by treatment with quinazoline or salicylate, inhibitors of nuclear factor-κB activation. Because MCP-1 is related to adipose inflammation and systemic insulin resistance, these results suggest that a reduction in adipose lipin-1 in obesity may exacerbate adipose inflammation and metabolism.
(Copyright © 2011 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: