The influence of peptide impurity profiles on functional tissue-organ bath response: the 11-mer peptide INSL6[151-161] case.

Autor: Verbeken M; Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, B-9000 Ghent, Belgium., Wynendaele E, Lefebvre RA, Goossens E, Spiegeleer BD
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2012 Feb 15; Vol. 421 (2), pp. 547-55. Date of Electronic Publication: 2011 Oct 06.
DOI: 10.1016/j.ab.2011.09.031
Abstrakt: Bioactive peptides have great pharmaceutical potential as nutraceuticals, diagnostics, and therapeutic drugs in several clinical areas. Thus, the search for novel lead peptides with a biological function has attracted renewed interest. Crude peptide material (i.e., ~70% purity) of INSL6[151-161] (NH₂-FRSLFWGNHSQ-COOH) was found to trigger a contractile response in guinea pig ileum longitudinal smooth muscle preparations using tissue-organ baths. However, the purified peptide (i.e., ≥ 95% purity) had no effect on this model. Further investigation with crude materials from other suppliers, with purities ranging between 50% and 80%, indicated that the crude products gave a false-positive functional tissue-organ bath conclusion. These observations question the functionality conclusions when using crude-purity peptide materials; during the initial research or discovery phase, peptide quality is generally neglected, possibly leading to misinterpretation of biological results due to by-products from peptide synthesis and, thus, wrong fail/pass decisions. Therefore, we strongly recommend appropriate quality control testing before using any peptides for initial biomedical research or discovery purposes.
(Copyright © 2011 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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