Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil.
| Autor: | Cantarelli V; Weinmann Laboratório, Porto Alegre, Brazil., Cavalcante B, Pilger DA, Souza F, Dias CG, Brodt T, Cantarelli M, Secchi C, d'Azevedo PA |
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| Jazyk: | angličtina |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2011 Oct; Vol. 44 (5), pp. 631-2. |
| DOI: | 10.1590/s0037-86822011000500021 |
| Abstrakt: | Introduction: Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). Methods: The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. Results: Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%. Conclusions: RT-PCR provides same day results, however it showed low sensitivity for VRE detection. |
| Databáze: | MEDLINE |
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