Chemical modification of botryosphaeran: structural characterization and anticoagulant activity of a water-soluble sulfonated (1-->3)(1-->6)-β-D-glucan.
| Autoři: | Brandi J; Departamento de Física, Química e Biologia, Faculdade de Ciências e Technologia, Universidade Estadual Paulista, CEP 19060-900, Presidente Prudente - São Paulo, Brazil., Oliveira ÉC, Monteiro N, Vasconcelos AF, Dekker RF, Barbosa AM, Silveira JL, Mourão PA, Corradi da Silva Mde L |
|---|---|
| Zdroj: | Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2011 Oct; Vol. 21 (10), pp. 1036-42. |
| Způsob vydávání: | Journal Article; Research Support, Non-U.S. Gov't |
| Jazyk: | English |
| Informace o časopise: | Publisher: Korean Society for Microbiology and Biotechnology Country of Publication: Korea (South) NLM ID: 9431852 Publication Model: Print Cited Medium: Internet ISSN: 1738-8872 (Electronic) Linking ISSN: 10177825 NLM ISO Abbreviation: J Microbiol Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2002- : Seoul : Korean Society for Microbiology and Biotechnology Original Publication: Seoul : Korean Society for Applied Microbiology, 1991-2001. |
| Výrazy ze slovníku MeSH: | Anticoagulants/*chemistry , Glucans/*chemistry, Anticoagulants/metabolism ; Anticoagulants/pharmacology ; Ascomycota/chemistry ; Ascomycota/metabolism ; Blood Coagulation/drug effects ; Glucans/metabolism ; Glucans/pharmacology ; Humans ; Molecular Structure ; Structure-Activity Relationship |
| Abstrakt: | The exopolysaccharide botryosphaeran (EPS(GLC); a (1--> 3)(1-->6)-β-D-glucan from Botryosphaeria rhodina MAMB- 05) was sulfonated to produce a water-soluble fraction (EPS(GLC)-S) using pyridine and chlorosulfonic acid in formamid. This procedure was then repeated twice to produce another fraction (EPSGLC-RS) with a higher degree of substitution (DS, 1.64). The purity of each botryosphaeran sample (unsulfonated and sulfonated) was assessed by gel filtration chromatography (Sepharose CL-4B), where each polysaccharide was eluted as a single symmetrical peak. The structures of the sulfonated and re-sulfonated botryosphaerans were investigated using ultraviolet-visible (UV-Vis), Fourier-transform infrared (FT-IR), and (13)C nuclear magnetic resonance ((13)C NMR) spectroscopies. EPS(GLC) and EPS(GLC)-RS were also assayed for anticoagulation activity, and EPS(GLC)-RS was identified as an anticoagulant. |
| Substance Nomenclature: | 0 (Anticoagulants) 0 (Glucans) 0 (botryosphaeran) |
| Entry Date(s): | Date Created: 20111028 Date Completed: 20120207 Latest Revision: 20190923 |
| Update Code: | 20240829 |
| DOI: | 10.4014/jmb.1105.05020 |
| PMID: | 22031027 |
| Autor: | Brandi J; Departamento de Física, Química e Biologia, Faculdade de Ciências e Technologia, Universidade Estadual Paulista, CEP 19060-900, Presidente Prudente - São Paulo, Brazil., Oliveira ÉC, Monteiro N, Vasconcelos AF, Dekker RF, Barbosa AM, Silveira JL, Mourão PA, Corradi da Silva Mde L |
| Jazyk: | angličtina |
| Zdroj: | Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2011 Oct; Vol. 21 (10), pp. 1036-42. |
| DOI: | 10.4014/jmb.1105.05020 |
| Abstrakt: | The exopolysaccharide botryosphaeran (EPS(GLC); a (1--> 3)(1-->6)-β-D-glucan from Botryosphaeria rhodina MAMB- 05) was sulfonated to produce a water-soluble fraction (EPS(GLC)-S) using pyridine and chlorosulfonic acid in formamid. This procedure was then repeated twice to produce another fraction (EPSGLC-RS) with a higher degree of substitution (DS, 1.64). The purity of each botryosphaeran sample (unsulfonated and sulfonated) was assessed by gel filtration chromatography (Sepharose CL-4B), where each polysaccharide was eluted as a single symmetrical peak. The structures of the sulfonated and re-sulfonated botryosphaerans were investigated using ultraviolet-visible (UV-Vis), Fourier-transform infrared (FT-IR), and (13)C nuclear magnetic resonance ((13)C NMR) spectroscopies. EPS(GLC) and EPS(GLC)-RS were also assayed for anticoagulation activity, and EPS(GLC)-RS was identified as an anticoagulant. |
| Databáze: | MEDLINE |
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