| Autor: |
Taniguchi M; Department of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan., Arikawa R, Kaedei Y, Tanihara F, Namula Z, Viet VL, Sato Y, Otoi T |
| Jazyk: |
angličtina |
| Zdroj: |
Cryo letters [Cryo Letters] 2011 Sep-Oct; Vol. 32 (5), pp. 410-4. |
| Abstrakt: |
Chemical toxicity of cryoprotectants to in vitro developmental competence of porcine oocytes was examined. In vitro-matured oocytes were exposed to 40 percent ethylene glycol (EG), glycerol (GLY), or 1,2-propanediol (PD), fertilized with spermatozoa, and cultured for 8 days. Compared to treatment with other cryoprotectants, exposure to EG resulted in the development of significantly more blastocysts, but the rate was significantly lower than that of non-exposed control oocytes. In vitro-matured oocytes were also equilibrated in 40 percent EG by 3 multi-step methods, after which their developmental competence was evaluated. The rate of blastocyst development was higher in the 4-step method than in the 2- and 3-step methods of equilibrium. These results indicate that cryoprotectants and equilibration methods affect the developmental competence of porcine oocytes and that EG may be a superior cryoprotectant for vitrification of these cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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