Reversal effect of intra-central amygdala microinjection of L-arginine on place aversion induced by naloxone in morphine conditioned rats.

Autor:	Karimi S; Dept. of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran., Karami M; Dept. of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran., Sahraei H; Dept. of Physiology and Biophysics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran., Rahimpour M; Dept. of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.
Jazyk:	angličtina
Zdroj:	Iranian biomedical journal [Iran Biomed J] 2011; Vol. 15 (3), pp. 92-9.
Abstrakt:	Background: Role of nitric oxide (NO) on expression of morphine conditioning using a solely classic task has been proposed previously. In this work, the involvement of NO on the expression of opioid-induced conditioning in the task paired with an injection of naloxone was investigated. Methods: Conditioning was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedure. Naloxone (0.05-0.4 mg/kg, i.p.), a selective antagonist of mu-opioid receptor, was administered once prior to morphine response testing. NO agents were administered directly into the central amygdala (CeA) prior to naloxone injection pre-testing. Results: Morphine (2.5-10 mg/kg, s.c.) produced a significant dose-dependent place preference in experimental animals. When naloxone (0.05-0.4 mg/kg, i.p.) was injected before testing of morphine (5 mg/kg, s.c.) response, the antagonist induced a significant aversion. This response was reversed due to injection of L-arginine (0.3-3 microg/rat), intra-CeA prior to naloxone administration. However, pre-injection of L-NAME (intra-CeA), an inhibitor of NO production, blocked this effect. Conclusion: The finding may reflect that NO in the nucleus participates in morphine plus naloxone interaction.
Databáze:	MEDLINE
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