| Autor: |
Daviso E; Leiden Institute of Chemistry, Einsteinweg 55, 2300 RA Leiden, The Netherlands., Janssen GJ, Alia A, Jeschke G, Matysik J, Tessari M |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of the American Chemical Society [J Am Chem Soc] 2011 Oct 26; Vol. 133 (42), pp. 16754-7. Date of Electronic Publication: 2011 Oct 05. |
| DOI: |
10.1021/ja206689t |
| Abstrakt: |
Several techniques rely on electron-nuclear interactions to boost the polarization of nuclear spins in the solid phase. Averaging out of anisotropic interactions as a result of molecular tumbling strongly reduces the applicability of such hyperpolarization approaches in liquids. Here we show for the first time that anisotropic electron-nuclear interactions in solution can survive sufficiently long to generate nuclear spin polarization by the solid-state photo-CIDNP mechanism. A 10,000-fold NMR signal increase in solution was observed for a giant biomolecular complex of a photosynthetic membrane protein with a tumbling correlation time in the submicrosecond regime, corresponding to a molecular weight close to 1 MDa. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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