| Autor: |
Findley SD; Center for Sustainable Energy, National Center for Soybean Biotechnology, Division of Plant Sciences, University of Missouri, Columbia, Missouri, USA., Mormile MR, Sommer-Hurley A, Zhang XC, Tipton P, Arnett K, Porter JH, Kerley M, Stacey G |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 2011 Nov; Vol. 77 (22), pp. 8106-13. Date of Electronic Publication: 2011 Sep 23. |
| DOI: |
10.1128/AEM.05925-11 |
| Abstrakt: |
The rumen, the foregut of herbivorous ruminant animals such as cattle, functions as a bioreactor to process complex plant material. Among the numerous and diverse microbes involved in ruminal digestion are the ruminal protozoans, which are single-celled, ciliated eukaryotic organisms. An activity-based screen was executed to identify genes encoding fibrolytic enzymes present in the metatranscriptome of a bovine ruminal protozoan-enriched cDNA expression library. Of the four novel genes identified, two were characterized in biochemical assays. Our results provide evidence for the effective use of functional metagenomics to retrieve novel enzymes from microbial populations that cannot be maintained in axenic cultures. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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