Detection of mammaglogin A in blood from breast cancer patients, before and after treatment, using a one-tube nested PCR protocol. Association with the absence of tumor estrogen receptors.

Autor: Ceballos MP; Area of Clinical Biochemistry, School of Biochemical and Pharmaceutical Sciences, National University of Rosario, Argentina., Zumoffen C, Massa E, Cipulli G, Funes CC, Gil AB, Morales C, Tozzini R, Ghersevich S
Jazyk: angličtina
Zdroj: Clinical biochemistry [Clin Biochem] 2011 Dec; Vol. 44 (17-18), pp. 1429-33. Date of Electronic Publication: 2011 Sep 12.
DOI: 10.1016/j.clinbiochem.2011.08.1140
Abstrakt: Objective: A one-tube nested RT-PCR protocol was set up and used to detect mammaglobin A (MGA) expression in blood samples from breast cancer patients. The correlation of MGA detection with prognostic factors was analyzed.
Design and Methods: Total RNA from nucleated blood cells was extracted from 65 breast cancer patients (before surgery and after the treatments) and 18 healthy subjects and used to detect MGA expression by a modified nested RT-PCR.
Results: MGA expression was detected in 38.4% of patients before surgery, and in 50% and 36.8% of post-treatment samples from patients that expressed MGA or were MGA negative before surgery, respectively. MGA detection was associated with the absence of tumor estrogen receptors (p=0.004).
Conclusions: MGA detection by the modified nested RT-PCR is a specific marker for circulating tumor cells in patients with breast carcinoma and a negative prognostic factor for the disease.
(Copyright © 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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