| Autor: |
Gommans WM; InteRNA Technologies BV, Utrecht, The Netherlands. gommans@interna-technologies.com, Berezikov E |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 786, pp. 167-78. |
| DOI: |
10.1007/978-1-61779-292-2_10 |
| Abstrakt: |
High-throughput sequencing has allowed for a comprehensive small RNA (sRNA) expression analysis of numerous tissues in a diverse set of organisms. The computational analysis of the millions of generated sequencing reads has led to the discovery of novel miRNAs and other sRNA species, and resulted in a better understanding of the roles these sRNAs play in development and disease. This chapter describes the generation of sRNA deep-sequencing libraries for the Illumina massively parallel sequencing platform by using a cloning method that anneals specific RNA sequences to the 5'- and 3'-ends of the sRNA molecules. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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