Nanopore sensors for nucleic acid analysis.

Autor: Venkatesan BM; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61820, USA., Bashir R
Jazyk: angličtina
Zdroj: Nature nanotechnology [Nat Nanotechnol] 2011 Sep 18; Vol. 6 (10), pp. 615-24. Date of Electronic Publication: 2011 Sep 18.
DOI: 10.1038/nnano.2011.129
Abstrakt: Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
Databáze: MEDLINE