Virulence modulation of Candida albicans biofilms by metal ions commonly released from orthodontic devices.

Autor: Ronsani MM; The Pontifical Catholic University of Parana, Department of Orthodontics, Brazil., Mores Rymovicz AU, Meira TM, Trindade Grégio AM, Guariza Filho O, Tanaka OM, Ribeiro Rosa EA
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2011 Dec; Vol. 51 (6), pp. 421-5. Date of Electronic Publication: 2011 Sep 10.
DOI: 10.1016/j.micpath.2011.08.007
Abstrakt: The installation of metal devices leads to an increase in the salivary concentration of metal ions and in the growth of salivary Candida spp. However, the relationship between released metal ions and Candida virulence has not been previously examined. The objective of this study was to evaluate whether metal ions affect fungal virulence. We prepared culture media containing Ni(2+), Fe(3+), Cr(3+), Co(2+) or a mixture of these metal ions at concentrations similar to those released in saliva of orthodontic patients. Biofilms of Candida albicans SC5314 were grown for 72 h and their biomasses were determined. The supernatants were analyzed for secretory aspartyl protease (SAP) and hemolysin activities. To verify changes in virulence following treatment with metals, proteolytic and hemolytic activities were converted into specific activities. The results revealed that all ions, except Co(2+), caused increases in biofilm biomass. In addition, Ni(2+) caused an increase in SAP activity and Fe(3+) reduced hemolytic activity. However, the SAP and hemolysin activities in the presence of the mixture of ions did not differ from those of control. These results indicate that metal ions released during the degradation of orthodontic appliances can modulate virulence factors in C. albicans biofilms.
(Copyright © 2011 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: