[Effect of BMP-2 on osteogenesis of bone mesenchymal stem cells in rats].

Autor:	Sun J; Department of Stomatology, Fudan University. Shanghai, China. Sunjian225@hotmail.com, Yu YC, Gu ZY, Gu L, Bi W
Jazyk:	čínština
Zdroj:	Shanghai kou qiang yi xue = Shanghai journal of stomatology [Shanghai Kou Qiang Yi Xue] 2011 Aug; Vol. 20 (4), pp. 352-7.
Abstrakt:	Purpose: To determine the effect of bone morphogenetic protein-2(BMP-2) on osteogenesis of bone mesenchymal stem cells(BMSCs) in rats. Methods: The primary culture of rat BMSCs was succeeded and then was identified. The rat BMSCs were infected with the recombinant lentivirus with BMP-2(Lenti-BMP-2). The osteogenic effect of BMP-2 was observed. In addition, adhesive ability of BMP-2-BMSCs was detected through adhesion assay and expression of osteogenic factors OPN,OCN,Col-I and smad was observed by RT-PCR and Western blot. SPSS11.0 software package was used for analysis. Results: Rat BMSCs were cultured and identified successfully. The osteogenic effect of BMSCs was improved by BMP-2. Lenti-BMP-2 BMSCs adhesive potential enhanced and osteogenic factors were up-regulated. Conclusions: BMP-2 may facilitate the osteogenic effect of the rat BMSCs and would provide favourable cell resource for tissue engineering.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu