| Autor: |
Andrade JP; Centro de Ciências Agrárias, Ambientais e Biológicas (CCAAB), Universidade Federal do Recôncavo da Bahia (UFRB), 44380-000 Cruz das Almas, BA, Brazil., Bispo AS, Marbach PA, do Nascimento RP |
| Jazyk: |
angličtina |
| Zdroj: |
Enzyme research [Enzyme Res] 2011; Vol. 2011, pp. 167248. Date of Electronic Publication: 2011 Aug 29. |
| DOI: |
10.4061/2011/167248 |
| Abstrakt: |
This study evaluated the production of cellulolytic enzymes by Trichoderma sp. IS-05 strain, isolated from sand dunes, according to its ability to grow on cellulose as carbon source. Wheat bran was tested as the carbon source and peptone tested as the nitrogen source. Different concentrations of carbon and nitrogen were tested using a factorial design to identify optimal cellulase activity production. The results showed that media containing wheat bran 4.0% (w/v) and peptone 0.25% (w/v) lead to the highest production, 564.0 U L(-1) of cellulase, obtained after 2 days of fermentation. The pH and temperature profile showed optimal activity at pH 3.0 and 60°C. As for thermostability, the cellulase was most tolerant at 60°C, retaining more than 59.6% of maximal activity even after 4 hours of incubation. The combination of acid pH, high temperature tolerance, and production of cellulase from agro-industrial residues by Trichoderma sp. IS-05 offers possibilities condition for the biomass hydrolysis process to produce bioethanol. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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