| Autor: |
Vikhrova MA, Batanova TA, Lebedev LR, Shingarova LN, Frank LA, Kirpichnikov MP, Tikunova NV |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 2011 May-Jun; Vol. 37 (3), pp. 334-43. |
| DOI: |
10.1134/s1068162011030198 |
| Abstrakt: |
Six unique phage antibodies to human TNF have been selected from a combinatorial library of human single chain fragment variable. ELISA and Western-blotting was used to study selected phage antibodies binding with TNF. The specificity of selected antibodies was determined by binding with interferon alpha and gamma, bovine serum albumin, ovalbumin and ubiquitin. Two antibodies, sA1 and sB3, were converted into a soluble single-chain antibody form and their affinity was 2.5 and 13.7 nM respectively. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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