Preferential recruitment of bone marrow-derived cells to rat palatal wounds but not to skin wounds.

Autor: Verstappen J; Department of Orthodontics and Craniofacial Biology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands. j.verstappen@dent.umcn.nl, van Rheden RE, Katsaros C, Torensma R, Von den Hoff JW
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2012 Jan; Vol. 57 (1), pp. 102-8. Date of Electronic Publication: 2011 Sep 03.
DOI: 10.1016/j.archoralbio.2011.08.005
Abstrakt: Objective: To investigate the contribution of bone marrow-derived cells to oral mucosa wounds and skin wounds.
Background: Bone marrow-derived cells are known to contribute to wound healing, and are able to differentiate in many different tissue-specific cell types. As wound healing in oral mucosa generally proceeds faster and with less scarring than in skin, we compared the bone marrow contribution in these two tissues.
Design: Bone marrow cells from GFP-transgenic rats were transplanted to irradiated wild-type rats. After recovery, 4-mm wounds were made in the mucoperiosteum or the skin. Two weeks later, wound tissue with adjacent normal tissue was stained for GFP-positive cells, myofibroblasts (a-smooth muscle actin), activated fibroblasts (HSP47), and myeloid cells (CD68).
Results: The fraction of GFP-positive cells in unwounded skin (19%) was larger than in unwounded mucoperiosteum (0.7%). Upon wounding, the fraction of GFP-positive cells in mucoperiosteum increased (8.1%), whilst it was unchanged in skin. About 7% of the myofibroblasts in both wounds were GFP-positive, 10% of the activated fibroblasts, and 25% of the myeloid cells.
Conclusions: The results indicate that bone marrow-derived cells are preferentially recruited to wounded oral mucosa but not to wounded skin. This might be related to the larger healing potential of oral mucosa.
(Copyright © 2011 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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