| Autor: |
Curry SR; Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA. currysr@upmc.edu, Schlackman JL, Hamilton TM, Henderson TK, Brown NT, Marsh JW, Shutt KA, Brooks MM, Pasculle AW, Muto CA, Harrison LH |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of clinical microbiology [J Clin Microbiol] 2011 Nov; Vol. 49 (11), pp. 3788-93. Date of Electronic Publication: 2011 Aug 31. |
| DOI: |
10.1128/JCM.00679-11 |
| Abstrakt: |
Active surveillance testing to identify and isolate asymptomatic carriers of toxigenic Clostridium difficile has been limited by the lack of a test that is sensitive, specific, and timely enough to serve as an infection control tool. We tested DNA preamplified from perirectal surveillance specimens in a liquid medium selective for C. difficile by using a modified commercial real-time PCR assay. All fermenting specimens were subcultured, and isolates were tested for toxigenicity. Culture-positive toxigenic isolates served as the gold standard for comparison with the broth preamplification/PCR assay. The limit of detection for the assay was 1 CFU. Relative to toxigenic anaerobic culture, the sensitivity, specificity, and positive and negative predictive values of this assay were 70/70 (100.0%), 422/426 (99.1%), 70/74 (94.6%), and 422/422 (100.0%), respectively. These data demonstrate that selective broth preamplification and real-time PCR of perirectal swab specimens constitute a practical approach to the detection of asymptomatic C. difficile carriage. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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