The involvement of the HO-1 pathway in the anti-inflammatory action of a sulfated polysaccharide isolated from the red seaweed Gracilaria birdiae.

Autor: de Sousa Oliveira Vanderlei E; Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, Fortaleza, CE, 60455-760, Brazil., de Araújo IW, Quinderé AL, Fontes BP, Eloy YR, Rodrigues JA, e Silva AA, Chaves HV, Jorge RJ, de Menezes DB, Evangelista JS, Bezerra MM, Benevides NM
Jazyk: angličtina
Zdroj: Inflammation research : official journal of the European Histamine Research Society ... [et al.] [Inflamm Res] 2011 Dec; Vol. 60 (12), pp. 1121-30. Date of Electronic Publication: 2011 Aug 31.
DOI: 10.1007/s00011-011-0376-8
Abstrakt: Objectives: The aim of this study was to investigate the involvement of the hemoxigenase-1 (HO-1) pathway in the anti-inflammatory action of a sulfated polysaccharide from the red seaweed Gracilaria birdiae (SP-Gb).
Methods: SP-Gb (5, 10 and 20 mg/kg) was administered to Wistar rats in a peritonitis model using carrageenan or a paw edema model using carrageenan or dextran. To analyze the involvement of HO-1 in the anti-inflammatory activity of SP-Gb, the animals were pretreated subcutaneously with a specific HO-1 inhibitor (ZnPP IX). To evaluate the systemic effects, SP-Gb (10 mg/kg) was administered to mice intraperitoneally before waiting for 48 h or for 14 days.
Results: SP-Gb (10 mg/kg) caused an anti-inflammatory effect that was evidenced by a decrease in leukocytes in the peritoneal cavity. SP-Gb also reduced the paw edema induced by carrageenan and inhibited the paw edema induced by dextran in the first half-hour. After being inhibited by ZnPP IX, the anti-inflammatory effect of SP-Gb on carrageenan-induced rat paw edema was not observed. SP-Gb did not cause mortality or significant changes in the biochemical, hematological and histopathological parameters.
Conclusion: SP-Gb may be used as a tool for further investigations into the inflammatory processes associated with the hemoxigenase-1 pathway.
Databáze: MEDLINE
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