| Autor: |
Burke DA; Gastroenterology Unit, General Infirmary, Leeds., Clayden SA, Axon AT |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of clinical pathology [J Clin Pathol] 1990 Apr; Vol. 43 (4), pp. 316-9. |
| DOI: |
10.1136/jcp.43.4.316 |
| Abstrakt: |
A microtitre method was developed to screen Escherichia coli from 48 patients with ulcerative colitis and 25 controls for serum resistance. Bactericidal resistance was indicated by a change in colour of indicator due to acid production by viable organisms and quantitated by a change in absorbance. The method clearly differentiated between organisms confirmed as resistant or sensitive by conventional techniques. Twenty four (50%) disease and 14 (56%) control E coli specimens showed serum resistance. Bactericidal competence of sera from patients with ulcerative colitis was assessed by incubating sensitive E coli with sera from 10 patients with ulcerative colitis and pooled normal serum. All sera effectively reduced viable counts to less than 6% of original inoculum. This study shows that serum samples from patients with ulcerative colitis are bactericidally competent and that there is no increase in the number of serum resistant E coli in patients with ulcerative colitis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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