[The use of photographic materials as a dry reagent for determining the peroxidase activity of biological preparations].

Autor: Aronbaev DM, Varfolomeev SD, Krivoruchko VI, Simonova MV
Jazyk: ruština
Zdroj: Meditsinskaia tekhnika [Med Tekh] 1990 Jan-Feb (1), pp. 39-40.
Abstrakt: A new method is reviewed for measuring the peroxidase activity of biopreparations using light quantum-sensitized photographic material as a detector. The method involves the combination of the reactions of peroxidase oxidation of hydroquinone, a constituent part of the developing solutions, by hydrogen peroxide and photochemical interaction of the remaining hydroquinone with silver halogenides of the photoemulsion layer. Visually, up to 2 ng/ml of horse radish peroxidase is detectable. On photometry of the spots obtained, the limit of peroxidase demonstration amounts to 0.6 ng/ml. The data obtained during measurements of the peroxidase activity of the saliva, blood serum, plant extracts and immunoenzymic conjugates are provided.
Databáze: MEDLINE