| Autor: |
Lim SF; Department of Physics, North Carolina State University, Raleigh, North Carolina 27695, USA., Karpusenko A, Sakon JJ, Hook JA, Lamar TA, Riehn R |
| Jazyk: |
angličtina |
| Zdroj: |
Biomicrofluidics [Biomicrofluidics] 2011 Sep; Vol. 5 (3), pp. 34106-341068. Date of Electronic Publication: 2011 Jul 25. |
| DOI: |
10.1063/1.3613671 |
| Abstrakt: |
We report the profiling of the 5-methyl cytosine distribution within single genomic-sized DNA molecules at a gene-relevant resolution. This method linearizes and stretches DNA molecules by confinement to channels with a dimension of about 250×200 nm(2). The methylation state is detected using fluorescently labeled methyl-CpG binding domain proteins (MBD), with high signal contrast and low background. DNA barcodes consisting of methylated and non-methylated segments are generated, with both short and long concatemers demonstrating spatially resolved MBD binding. The resolution of the technique is better than 10 kbp, and single-molecule read-lengths exceeding 140 kbp have been achieved. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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