| Autor: |
Mahadeva Swamy HM; Bio Pesticide Laboratory, Division of Biotechnology, Indian Institute of Horticultural Research, Hessarghatta Lake Post, Bangalore, 560089 Karnataka, India., Asokan R, Nagesha SN, Arora DK, Birah A, Mahmood R |
| Jazyk: |
angličtina |
| Zdroj: |
Current microbiology [Curr Microbiol] 2011 Nov; Vol. 63 (5), pp. 420-5. Date of Electronic Publication: 2011 Aug 21. |
| DOI: |
10.1007/s00284-011-9998-x |
| Abstrakt: |
Bt strains were isolated from soils of Andaman and Nicobar Islands and characterized by microscopic and molecular methods. Diversity was observed both in protein and cry gene profiles, where majority of the isolates showed presence of 65 kDa protein band on SDS-PAGE while rest of them showed 130, 72, 44, and 29 kDa bands. PCR analysis revealed predominance of cry1I and cry7, 8 genes in these isolates. The PCR screening strategy presented here led us to identify putative novel cry genes which could be active against Coleoptera insects. Variation in the nucleotide sequences of cry genes from the isolates suggests that the genetic diversity of Bt isolates results from the influence of different ecological factors and spatial separation between strains generated by the conquest of different habitats in the soils of Andaman and Nicobar islands. The implications of our studies are important from the point of view of identifying novel cry genes that could be toxic to insects other than lepidoptera. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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