Quantitation of infectious myonecrosis virus in different tissues of naturally infected Pacific white shrimp, Litopenaeus vannamei, using real-time PCR with SYBR Green chemistry.

Autor: da Silva SM; Departamento de Pesca e Aqüicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, 52171-900 Dois Irmãos, Recife, Pernambuco, Brazil., Pinheiro AC, Coimbra MR
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2011 Nov; Vol. 177 (2), pp. 197-201. Date of Electronic Publication: 2011 Aug 09.
DOI: 10.1016/j.jviromet.2011.08.001
Abstrakt: The Pacific white shrimp, Litopenaeus vannamei, is the most important shrimp species in volume in world aquaculture. However, in recent decades, outbreaks of diseases, especially viral diseases, have led to significant economic losses, threatening the sustainability of shrimp farming worldwide. In 2004, Brazilian shrimp farming was seriously affected by a new disease caused by the Infectious myonecrosis virus (IMNV). Thus, disease control based on rapid and sensitive pathogen detection methods has become a priority. In this study, a specific quantitation method for IMNV was developed using real-time PCR with SYBR Green chemistry and viral load of the principal target tissues of chronically infected animals was quantified. The quantitative analysis revealed that mean viral load ranged from 5.08×10(8) to 1.33×10(6)copies/μg of total RNA in the hemolymph, 5.096×10(5) to 1.26×10(3)copies/μg in the pleopods, 6.85×10(8) to 3.09×10(4)copies/μg in muscle and 8.15×10(6) to 3.90×10(3)copies/μg in gills. Different viral loads of IMNV were found with greater values in the hemolymph and muscle, followed by the pleopods and gills.
(Copyright © 2011 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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