| Autor: |
Boligon AA; Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Build 26, room 1115, Santa Maria, CEP 97105-900, Brazil. alineboligon@yahoo.com.br, Janovik V, Frohlich JK, Spader TB, Froeder AL, Alves SH, Athayde ML |
| Jazyk: |
angličtina |
| Zdroj: |
Natural product research [Nat Prod Res] 2012; Vol. 26 (10), pp. 939-44. Date of Electronic Publication: 2011 Aug 11. |
| DOI: |
10.1080/14786419.2010.535151 |
| Abstrakt: |
The antimicrobial and cytotoxic activities of the dichloromethane, ethyl acetate and butanolic fractions from the leaves, twigs and stem bark of Scutia buxifolia were evaluated using the broth microdilution method and the brine shrimp lethality method, respectively. Phytochemical analysis was performed by thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The antimicrobial results demonstrated that the strongest effect occurred with the butanol fraction from the twigs and the ethyl acetate fraction from the stem bark against Saccharomyces cerevisiae (minimal inhibitory concentration; MIC = 62.5 µg mL(-1)), whereas the ethyl acetate and butanolic fractions from the twigs and stem bark were effective against Pseudomonas aeruginosa and Staphylococcus aureus, with MIC values ranging from 125 to 500 µg mL(-1). LD(50) values varied from 50.00 ± 0.22 to 82.23 ± 0.34 µg mL(-1). Quercetin, quercitrin, isoquercitrin and rutin were identified by HPLC and may be partially responsible for the antimicrobial activities observed. This study reports for the first time the antimicrobial and cytotoxic activities of S. buxifolia leaves, twigs and stem bark. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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