An LC-MS/MS method for simultaneous determination of vincristine and verapamil in rat plasma after oral administration of a dual agent formulation.

Autor: Ling G; School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China., Zhang P, Sun J, Zhang W, Fu Q, Zhang T, Deng Y, He Z
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2011 Sep; Vol. 25 (9), pp. 963-9. Date of Electronic Publication: 2010 Dec 10.
DOI: 10.1002/bmc.1565
Abstrakt: A rapid and sensitive method for simultaneous determination of vincristine and verapamil in rat plasma was first developed and validated, using high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) in multiple reaction monitoring (MRM) mode via electrospray ionization (ESI). The method, which required a small sample volume (25 µL) of plasma, was linear in the concentration range of 0.5-500 ng/mL for vincristine and 0.1-100.0 ng/mL for verapamil. Finally, the method was successfully employed in a pharmacokinetic study of vincristine and verapamil in rats after an oral administration of a dual-agent formulation containing vincristine and verapamil.
(Copyright © 2010 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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