DNA damage induction in human cells exposed to vanadium oxides in vitro.

Autor: Rodríguez-Mercado JJ; Unidad de Investigación en Genética y Toxicología Ambiental (UNIGEN), Laboratorio L5-PA, Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z), Facultad de Estudios Superiores-Zaragoza, Campus II, UNAM, A.P. 9-020, C.P. 15000, México, D.F., Mexico., Mateos-Nava RA, Altamirano-Lozano MA
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2011 Dec; Vol. 25 (8), pp. 1996-2002. Date of Electronic Publication: 2011 Jul 22.
DOI: 10.1016/j.tiv.2011.07.009
Abstrakt: Vanadium and vanadium salts cause genotoxicity and elicit variable biological effects depending on several factors. In the present study, we analyzed and compared the DNA damage and repair processes induced by vanadium in three oxidation states. We used human blood leukocytes in vitro and in a single cell gel electrophoresis assay at two pH values. We observed that vanadium(III) trioxide and vanadium(V) pentoxide produced DNA single-strand breaks at all of the concentrations (1, 2, 4, or 8 μg/ml) and treatment times (2, 4, or 6 h) tested. Vanadium(IV) tetraoxide treatment significantly increased DNA damage at all concentrations for 4 or 6 h of treatment but not for 2 h of treatment. The DNA repair kinetics indicated that most of the cells exposed to vanadium III and V for 4 h recovered within the repair incubation time of 90 min; however, those exposed to vanadium(IV) repaired their DNA within 120 min. The data at pH 9 indicated that vanadium(IV) tetraoxide induced DNA double-strand breaks. Our results show that the genotoxic effect of vanadium can be produced by any of its three oxidation states. However, vanadium(IV) induces double-strand breaks, and it is known that these lesions are linked with forming structural chromosomal aberrations.
(Copyright © 2011 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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