| Autor: |
Whalen M; Crop Improvement and Utilization Unit, Western Regional Research Center, ARS USDA, 800 Buchanan Street, Albany, CA 94710, US., Richter T, Zakhareyvich K, Yoshikawa M, Al-Azzeh D, Adefioye A, Spicer G, Mendoza LL, Morales CQ, Klassen V, Perez-Baron G, Toebe CS, Tzovolous A, Gerstman E, Evans E, Thompson C, Lopez M, Ronald PC |
| Jazyk: |
angličtina |
| Zdroj: |
Physiological and molecular plant pathology [Physiol Mol Plant Pathol] 2008 Jan; Vol. 72 (1-3), pp. 46-55. |
| DOI: |
10.1016/j.pmpp.2008.05.006 |
| Abstrakt: |
AvrRxv is a member of a family of pathogen effectors present in pathogens of both plant and mammalian species. Xanthomonas campestris pv. vesicatoria strains carrying AvrRxv induce a hypersensitive response (HR) in the tomato cultivar Hawaii 7998. Using a yeast two-hybrid screen, we identified a 14-3-3 protein from tomato that interacts with AvrRxv called AvrRxv Interactor 1 (ARI1). The interaction was confirmed in vitro with affinity chromatography. Using mutagenesis, we identified a 14-3-3-binding domain in AvrRxv and demonstrated that a mutant in that domain showed concomitant loss of interaction with ARI1 and HR-inducing activity in tomato. These results demonstrate that the AvrRxv bacterial effector recruits 14-3-3 proteins for its function within host cells. AvrRxv homologues YopP and YopJ from Yersinia do not have AvrRxv-specific HR-inducing activity when delivered into tomato host cells by Agrobacterium. Although YopP itself cannot induce HR, its C-terminal domain containing the catalytic residues can replace that of AvrRxv in an AvrRxv-YopP chimera for HR-inducing activity. Phylogenetic analysis indicates that the sequences encoding the C-termini of family members are evolving independently from those encoding the N-termini. Our results support a model in which there are three functional domains in proteins of the family, translocation, interaction, and catalytic. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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