Phage display identifies two Caprine Arthritis Encephalitis Virus env epitopes.
| Autor: | Gazarian K; Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), México D,F,, Ciudad Universitaria, 04510, México. karlen@servidor.unam.mx., Setién AA, Gazarian T, Pierle SA |
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| Jazyk: | angličtina |
| Zdroj: | Veterinary research [Vet Res] 2011 Jul 22; Vol. 42, pp. 87. Date of Electronic Publication: 2011 Jul 22. |
| DOI: | 10.1186/1297-9716-42-87 |
| Abstrakt: | Using phage display and IgG of a goat infected with Caprine Arthritis Encephalitis Virus (CAEV) we obtained families of 7 mer constrained peptides with consensus motifs LxSDPF/Y and SWN/KHWSY and mapped the epitopes mimicked by them at the Env 6-LISDPY-11 and 67-WNTYHW-72 sites of the mature gp135 amino acid sequence. The first epitope fell into the N-terminal immunogenic aa1-EDYTLISDPYGFS- aa14 site identified previously with a synthetic peptide approach; the second epitope has not been described previously. The first epitope is mostly conserved across CAEV isolates whereas the second newly described epitope is extremely conserved in Small Ruminant Lentiviruses env sequences. As being immunodominant, the epitopes are candidate targets for mimotope-mediated diagnosis and/or neutralization. |
| Databáze: | MEDLINE |
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