| Autor: |
Valeeva OA, Pastushkov LKh, Pakharukova NA, Dobrokhotov IV, Larina IM |
| Jazyk: |
ruština |
| Zdroj: |
Fiziologiia cheloveka [Fiziol Cheloveka] 2011 May-Jun; Vol. 37 (3), pp. 98-102. |
| Abstrakt: |
Human proteome is very plastic, it changes under the influence of various biological factors. It is of big interest to find out how specific factors of an environment, including a long-term isolation affect on urine proteome. The study was conducted during the experiment with 105-day isolation. In the present investigation we collected urine samples from 6 healthy volunteers (26-41 years old). The physical activity, daily rhythms and diet were controlled. Urine samples were fractionated on magnetic beads MB-HIC C8 (MB - hydrophobic-interaction chromatography) with ClinProt robot (Bruker Daltonics) prior to MALDI-TOF mass spectrometer analysis with Autoflex III TOF/TOF (Bruker Daltonics), working in a positive linear mode. 117 peaks have been obtained in each spectrum of urine. We have shown that even during isolation and under controlled conditions of life a high variability of urine proteome of healthy personas (36 protein MC-peaks in the urine, on average) are revealed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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