Improvement of a PCR test to diagnose infection by Mansonella ozzardi.
| Autor: | Vera LJ; Coordenação de Medicina, Faculdade São Lucas, Porto Velho, RO, Brasil., Basano Sde A, Camargo Jde S, França AK, Ferreira Rde G, Casseb AA, Medeiros JF, Fontes G, Camargo LM |
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| Jazyk: | angličtina |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2011 May-Jun; Vol. 44 (3), pp. 380-2. |
| DOI: | 10.1590/s0037-86822011000300023 |
| Abstrakt: | Introduction: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. Methods: In order to increase diagnosis efficacy, the PCR technique was improved. Results and Conclusions: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears. |
| Databáze: | MEDLINE |
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