| Autor: |
Yang ZF; Clinical Virology Division, State Key Laboratory of Respiratory Diseases, Guangzhou Medical University, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China. jeffyah@163.com, Wang YT, Qin S, Zhao SS, Zhao YS, Lin Q, Guan WD, Huang QD, Mo ZY, Li CY, Zhong NS |
| Jazyk: |
čínština |
| Zdroj: |
Bing du xue bao = Chinese journal of virology [Bing Du Xue Bao] 2011 May; Vol. 27 (3), pp. 218-23. |
| Abstrakt: |
This study was to investigate the antiviral effects of a hot water soluble extract S-03 isolated from Isatis indigotica root on different subtypes of influenza A and B viruses in MDCK cell cultures, using plaque reduction, immunofluorescence and hemo-agglutination inhibition (HAD) assays. Chemical analysis of the extract S-03 showed that it contained high proportion of polysaccharides. The antiviral effects in vitro showed that the S-03 had no effect on different influenza viruses if the drug was used before virus adsorption, but S-03 showed obvious activities against influenza viruses if treatment after virus adsorption or direct reaction of drug and virus before virus adsorption. Hemagglutination inhibition assay showed that S-03 inhibited HA activities of different human influenza viruses (inhibition concentration ranged from 3.12 to 25 mg/mL), avain influenza viruses (inhibition concentration ranged from 25 to 50 mg/mL). The antiviral effects of S-03 on different influenza A and B viruses in vitro might be through the inhibition of the HA to prevent infection. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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