| Autor: |
Escote-Carlson LJ; Biotechnology Laboratory, University of British Columbia, Vancouver, Canada., Gabay-Laughnan S, Laughnan JR |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular & general genetics : MGG [Mol Gen Genet] 1990 Sep; Vol. 223 (3), pp. 457-64. |
| DOI: |
10.1007/BF00264454 |
| Abstrakt: |
A WF9 strain of maize with the RD subtype of the S male-sterile cytoplasm (CMS-S) was converted to the inbred M825 nuclear background by recurrent backcrossing. The organization of the mitochondrial genomes of the F1 and succeeding backcross progenies was analyzed and compared with the progenitor RD-WF9 using probes derived from the S1 and S2 mitochondrial episomes, and probes containing the genes for cytochrome c oxidase subunit I (coxI), cytochrome c oxidase subunit II (coxII) and apocytochrome b (cob). Changes in mitochondrial DNA (mtDNA) organization were observed for S1-, S2-, and coxI-homologous sequences that involve loss of homologous restriction enzyme fragments present in the RD-WF9 progenitor. With the coxI probe, the loss of certain fragments was accompanied by the appearance of a fragment not detectable in the progenitor. The changes observed indicate the effect of the nuclear genome on the differential replication of specific mitochondrial subgenomic entities. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink