Quantification of the Aspergillus versicolor allergen in house dust.

Autor: Shi C; Ottawa-Carleton Institute of Chemistry, Carleton University, Ottawa, ON K1S5B6, Canada., Belisle D, Miller JD
Jazyk: angličtina
Zdroj: Journal of immunological methods [J Immunol Methods] 2011 Sep 30; Vol. 372 (1-2), pp. 89-94. Date of Electronic Publication: 2011 Jul 13.
DOI: 10.1016/j.jim.2011.06.034
Abstrakt: Aspergillus versicolor, a fungus commonly found on damp building materials, produces the allergen, Asp v 13. Here we report a sensitive Asp v 13 capture ELISA for A. versicolor spores and spore- and mycelial fragments in house dust samples. The method is based on a double polyclonal capture ELSIA. The detection limits for Asp v 13 antigen and A. versicolor spores without dust were 2.44 pg and 12 ng (ca. 110 spores). Detection limits for Asp v 13 and A. versicolor spores in sieved house dust samples were 1.0 ng and 7.8 μg per gram dry weight house dust, respectively. This detection limit is lower than for other house dust allergen immunoassays including for Stachybotrys chartarum, Aspergillus fumigatus, but much lower than that from Alternaria alternata.
(Copyright © 2011 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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