| Autor: |
Jonigk D; Medizinische Hochschule Hannover, Institute of Pathology, Hannover, Germany., Modde F, Bockmeyer CL, Becker JU, Lehmann U |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2011; Vol. 755, pp. 67-75. |
| DOI: |
10.1007/978-1-61779-163-5_5 |
| Abstrakt: |
mRNA extraction and subsequent RT-polymerase chain reaction (PCR)-based expression analysis from laser-microdissected material is by now a well-established and reproducible method. Most routinely stored tissue samples are preserved as formalin-fixed, paraffin-embedded materials. While this allows for a convenient storage and stable preservation of nucleic acids, deparaffinization before staining for laser microdissection may result in a significant loss of mRNA quality and consequently of PCR sensitivity. We describe a method of isolating anatomic compartments from non-deparaffinized, formalin-fixed, and paraffin-embedded tissues by laser-assisted microdissection which allows for a highly efficient mRNA retrieval. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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