| Autor: |
Rose RC; Department of Medicine, University of Rochester School of Medicine, New York 14642., Bonnez W, Strike DG, Reichman RC |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of general virology [J Gen Virol] 1990 Nov; Vol. 71 ( Pt 11), pp. 2725-9. |
| DOI: |
10.1099/0022-1317-71-11-2725 |
| Abstrakt: |
The L2 open reading frames (ORFs) of human papillomavirus (HPV) types 6b and 11 were expressed as full-length non-fusion proteins in Spodoptera frugiperda (Sf-9) cells using recombinant baculovirus. Both proteins were detected on Western blots as immunoreactive bands which migrated with apparent Mrs of 76K and 78K, respectively, and contained both cross-reactive and type-specific epitopes, as determined by polyclonal antisera directed against defined subregions of the HPV-6b and HPV-11 L2 ORFs. In addition, the minor capsid protein of HPV-11 particles co-migrates with the HPV-11 L2 ORF product and is immunoreactive with HPV-11 L2-specific antisera. These observations indicate that the anomalous electrophoretic mobilities of papillomavirus L2 ORF proteins can be explained without invoking post-transcriptional processing events and that the minor capsid protein of HPV-11 is antigenically and biophysically related to the HPV-11 L2 ORF product. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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