Enhanced production of plasmid DNA by engineered Escherichia coli strains.

Autor: Pablos TE; Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Artificios No. 40, Col. Miguel Hidalgo, Del. Álvaro Obregón, México DF, CP 01120, Mexico., Soto R, Mora EM, Le Borgne S, Ramírez OT, Gosset G, Lara AR
Jazyk: angličtina
Zdroj: Journal of biotechnology [J Biotechnol] 2012 Apr 30; Vol. 158 (4), pp. 211-4. Date of Electronic Publication: 2011 Jun 17.
DOI: 10.1016/j.jbiotec.2011.04.015
Abstrakt: Escherichia coli strains VH33 (PTS⁻ GalP⁺ strain displaying a strongly reduced overflow metabolism) and VH34 (additionally lacking the pyruvate kinase A) were evaluated for the production of a plasmid DNA (pDNA) vaccine. The parent (W3110) and mutant strains were cultured using 10 g of glucose/L. While the specific growth rates of the three strains were similar, they presented differences in the accumulation of acetate. W3110 accumulated up to 4 g/L of acetate, VH33 produced 1.4 g/L, and VH34 only 0.78 g/L. VH33 and VH34 produced 76% and 300% more pDNA than W3110. Moreover, VH34 demanded 33% less oxygen than VH33 and W3110, which can be advantageous for large-scale applications.
(Copyright © 2011 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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