The zymogen-enteropeptidase system: A practical approach to study the regulation of enzyme activity by proteolytic cleavage*.

Autor: Pizauro JM Jr; Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP, 14884-900 Jaboticabal SP, Brazil. jpizauro@fcav.unesp.br., Ferro JA, de Lima AC, Routman KS, Portella MC
Jazyk: angličtina
Zdroj: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology [Biochem Mol Biol Educ] 2004 Jan; Vol. 32 (1), pp. 45-8.
DOI: 10.1002/bmb.2004.494032010311
Abstrakt: The present research describes an efficient procedure to obtain high levels of trypsinogen and chymotrypsinogen by using a simple, rapid, and easily reproducible method. The extraction process and the time-course of activation of zymogens can be carried out in a single laboratory period, without sophisticated equipment. The main objective was to prepare a laboratory class that would stimulate student interest in enzyme regulation, exploring the fact that the catalytic activity of some enzymes is regulated by different mechanisms. The regulation of proteolytic enzymes requires the synthesis of an inactive zymogen and its being irreversibly "switched on" by specific proteolytic cleavage.
(Copyright © 2004 International Union of Biochemistry and Molecular Biology, Inc.)
Databáze: MEDLINE
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