Sequence-specific binding to telomeric DNA is not a conserved property of the Cdc13 DNA binding domain.
| Autor: | Mandell EK; Salk Institute for Biological Studies, La Jolla, California 92037, USA., Gelinas AD, Wuttke DS, Lundblad V |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Biochemistry [Biochemistry] 2011 Jul 26; Vol. 50 (29), pp. 6289-91. Date of Electronic Publication: 2011 Jun 29. |
| DOI: | 10.1021/bi2005448 |
| Abstrakt: | In the budding yeast Saccharomyces cerevisiae, chromosome end protection is provided by a heterotrimeric complex composed of Cdc13 in association with the RPA-like proteins Stn1 and Ten1. We report here that the high affinity and specificity of the S. cerevisiae Cdc13 DNA binding domain for single-stranded telomeric DNA are not widely shared by other fungal Cdc13 proteins, suggesting that restriction of this complex to telomeres may be limited to the Saccharomyces clade. We propose that the evolutionarily conserved task of Stn1 and Ten1 (and their associated large subunit) is a genome-wide role in DNA replication rather than a telomere-dedicated activity. (© 2011 American Chemical Society) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|