Thiosemicarbazone derivate protects from AAPH and Cu2+ -induced LDL oxidation.

Autor: Barcelos RP; Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus UFSM, Santa Maria, RS, Brazil., Portella Rde L, da Rosa EJ, Fonseca Ade S, Bresolin L, Carratu V, Soares FA, Barbosa NV
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2011 Jul 04; Vol. 89 (1-2), pp. 20-8. Date of Electronic Publication: 2011 May 19.
DOI: 10.1016/j.lfs.2011.04.026
Abstrakt: Aims: Several lines of evidence support the hypotheses that the oxidation of low density lipoprotein (LDL) may play a crucial role in the initiation and progression of atherosclerosis. Oxidative stress is one of the causes of the overproduction of reactive species that increase the formation of oxidized LDL. Thiosemicarbazones are compounds used in anticancer, antiviral and antifungal therapy; however, its redox activity has been controversial. Thus, we tested, in vitro, a possible antioxidant activity of a thiosemicarbazone derivate, the isatin-3-N(4)-benzilthiosemicarbazone (IBTC).
Main Methods: We measured the conjugated diene formation in serum and LDL as well as the loss of tryptophan fluorescence in LDL induced by two oxidant agents, 2,2-azobis(2-amidinopropane dihydrochloride) (AAPH) and Cu(2+). Thiobarbituric acid reactive substances (TBARS) formation in LDL and in different rat tissues was also assessed. The toxicity of IBTC was measured using aortic slices viability assay.
Key Findings: Our results show that IBTC significantly reduced the AAPH and Cu(2+)-induced formation of conjugated dienes, increased in a dose-dependent manner the lag phase and the t(1/2) of tryptophan fluorescence, and reduced the TBARS formation in LDL, plasma and rat tissues, showing no toxicity to aortic slices.
Significance: These results indicate that IBTC is a good antioxidant and a promising antiatherogenic agent for further studies in vivo.
(Copyright © 2011 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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