Interleukin-28B genotyping by melt-mismatch amplification mutation assay PCR analysis using single nucleotide polymorphisms rs12979860 and rs8099917, a useful tool for prediction of therapy response in hepatitis C patients.

Autor: Fonseca-Coronado S; Unidad de Investigación Multidisciplinaria, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico City, Mexico., Vaughan G, Cruz-Rivera MY, Carpio-Pedroza JC, Ruiz-Tovar K, Ruiz-Pacheco JA, Escobar-Gutiérrez A
Jazyk: angličtina
Zdroj: Journal of clinical microbiology [J Clin Microbiol] 2011 Jul; Vol. 49 (7), pp. 2706-10. Date of Electronic Publication: 2011 May 25.
DOI: 10.1128/JCM.00877-11
Abstrakt: Several studies have identified associations between single nucleotide polymorphisms (SNPs) occurring near the interleukin-28B (IL-28B) gene and response to antiviral treatment among hepatitis C virus (HCV) patients. Here, we describe a reliable melt-mismatch amplification mutation assay (melt-MAMA) PCR-based genotyping method for IL-28B which can be used in the management of HCV patients, helping to better define the course of therapy.
Databáze: MEDLINE