| Autor: |
Lecault V; Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada., Vaninsberghe M, Sekulovic S, Knapp DJ, Wohrer S, Bowden W, Viel F, McLaughlin T, Jarandehei A, Miller M, Falconnet D, White AK, Kent DG, Copley MR, Taghipour F, Eaves CJ, Humphries RK, Piret JM, Hansen CL |
| Jazyk: |
angličtina |
| Zdroj: |
Nature methods [Nat Methods] 2011 May 22; Vol. 8 (7), pp. 581-6. Date of Electronic Publication: 2011 May 22. |
| DOI: |
10.1038/nmeth.1614 |
| Abstrakt: |
Heterogeneity in cell populations poses a major obstacle to understanding complex biological processes. Here we present a microfluidic platform containing thousands of nanoliter-scale chambers suitable for live-cell imaging studies of clonal cultures of nonadherent cells with precise control of the conditions, capabilities for in situ immunostaining and recovery of viable cells. We show that this platform mimics conventional cultures in reproducing the responses of various types of primitive mouse hematopoietic cells with retention of their functional properties, as demonstrated by subsequent in vitro and in vivo (transplantation) assays of recovered cells. The automated medium exchange of this system made it possible to define when Steel factor stimulation is first required by adult hematopoietic stem cells in vitro as the point of exit from quiescence. This technology will offer many new avenues to interrogate otherwise inaccessible mechanisms governing mammalian cell growth and fate decisions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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