[Study on the identification characteristics of rRNA genes on Yersinia pestis].

Autor:	Zhang HJ; State Key Laboratory for Infectious Disease Prevention and Control, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China., Wei JC, Zhang EM, Shen XN, Liang Y, Zhu XY, Xu DL, Sun LN, Zhang JH, Yu DZ, Hai R
Jazyk:	čínština
Zdroj:	Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi [Zhonghua Liu Xing Bing Xue Za Zhi] 2011 Apr; Vol. 32 (4), pp. 366-9.
Abstrakt:	Objective: To study the identification characteristics of rRNA genes on Yersinia (Y.) pestis. Methods: By means of comparative genomics, we compared the rRNA genome sequences of nine completely sequenced strains of Y. pestis isolated from China and other countries by Clustal W software. We also compared the 2000 bp sequence adjacent to the rRNA genes, rRNA genes and 16S-23S rRNA spacer region respectively to determine the identification features of rRNA genes for Y. pestis. Results: There were 6 rRNA gene clusters in the strains of D182038, D106004, Z176003 and CO92 respectively (6 copies strain). There were 7 rRNA gene clusters in the strains of 91001, KIM, Nepal516, Antiqua and Pestoides F (7 copies strain). According to the 2000 bp sequence, 13 types of rRNA gene clusters could classify the strains between the 6 copies and 7 copies. There were 4 types of tRNA gene among the 16S-23S rRNA spacer region that could classify the strains among the 6 copies and 7 copies strains respectively. The number of point mutation among the 23S rRNA gene was statistically different in some copies under ANOVA analysis (F = 0.548, P = 0.815 > 0.05 among the strains and F = 5.228, P < 0.01 among the copies). Conclusion: The 2000 bp sequence adjacent to the rRNA genes, tRNA gene and 23S rRNA gene sequence could serve as the identification sign of rRNA genes for classifing the strains of Y. pestis.
Databáze:	MEDLINE
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