| Autor: |
Ermakova II; Department of Cell Cultures, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia. irinabiochem@mail.ru, Sakuta GA, Potekhina MA, Fedorova MA, Hoffmann R, Morozov VI |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2011 Mar; Vol. 76 (3), pp. 359-65. |
| DOI: |
10.1134/s0006297911030102 |
| Abstrakt: |
The major proteoglycans from L6J1 rat myoblast culture were identified. The proteoglycans were isolated from different constituents of cell culture: culture medium, extracellular matrix (ECM), and myoblasts. To identify their core proteins, the proteoglycans were treated with enzymes specifically digesting chondroitin/dermatan sulfates or chondroitin sulfates. Subsequent electrophoresis and mass spectrometry revealed versican, collagen XII, and inter-α-trypsin inhibitor classified as chondroitin sulfate proteoglycans and biglycan known to be chondroitin/dermatan sulfate proteoglycan. Versican was identified in ECM and the other proteoglycans in the culture medium. Such difference in localization is likely to be a consequence of different biological functions. Versican, collagen XII, and biglycan are synthesized by myoblasts and inter-α-trypsin inhibitor originates from fetal bovine serum (a culture medium component). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
