| Autor: |
Borisova SA; Institute for Genomic Biology, Howard Hughes Medical Institute University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA., Christman HD, Metcalf ME, Zulkepli NA, Zhang JK, van der Donk WA, Metcalf WW |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of biological chemistry [J Biol Chem] 2011 Jun 24; Vol. 286 (25), pp. 22283-90. Date of Electronic Publication: 2011 May 04. |
| DOI: |
10.1074/jbc.M111.237735 |
| Abstrakt: |
A variety of microorganisms have the ability to use phosphonic acids as sole sources of phosphorus. Here, a novel pathway for degradation of 2-aminoethylphosphonate in the bacterium Sinorhizobium meliloti 1021 is proposed based on the analysis of the genome sequence. Gene deletion experiments confirmed the involvement of the locus containing phnW, phnA, and phnY genes in the conversion of 2-aminoethylphosphonate to inorganic phosphate. Biochemical studies of the recombinant PhnY and PhnA proteins verified their roles as phosphonoacetaldehyde dehydrogenase and phosphonoacetate hydrolase, respectively. This pathway is likely not limited to S. meliloti as suggested by the presence of homologous gene clusters in other bacterial genomes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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