Modulation of mature B cells in mice following treatment with ouabain.

Autor: de Paiva LS; Laboratório de Imunorregulação, Instituto de Biologia, Departamento de Imunobiologia, Universidade Federal Fluminense, Niterói, RJ, Brazil. luspaiva@yahoo.com.br, Costa KM, Canto FB, Cabral VR, Fucs R, Nobrega A, Rumjanek VM
Jazyk: angličtina
Zdroj: Immunobiology [Immunobiology] 2011 Sep; Vol. 216 (9), pp. 1038-43. Date of Electronic Publication: 2011 Mar 12.
DOI: 10.1016/j.imbio.2011.03.002
Abstrakt: Ouabain (OUA) is an endogenous hormone released by the adrenal gland under stress situations. Steroid hormones and glucocorticoids have been characterized as selective inhibitors of lymphopoiesis. The present report shows in vivo modulation of mature B cells in bone marrow, spleen and peripheral blood by ouabain. Mice injected intraperitonially (i.p.) with ouabain 0.56 mg/kg for 3 consecutive days displayed, 24 h after last injection, a decreased cellularity in the bone marrow with diminution of the mature B cell subpopulation while the other B cell subpopulations were preserved. Percentually, the myeloid lineage in bone marrow was increased by ouabain. Numbers of mature B lymphocytes in spleen and peripheral blood were reduced following in vivo treatment. In vitro, the B cell populations were not affected. The effects appear to be independent of steroid hormones and strain. The presence of stable levels of glucocorticoids seems to be important because the effects could only be observed from the fourth week animal's life, when glucocorticoid levels are stable. These results open new perspectives for a potential use of ouabain as an immunomodulator.
(Copyright © 2011 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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