Proteome interrogation using nanoprobes to identify targets of a cancer-killing molecule.
| Autor: | Li L; Key Laboratory of Colloid and Interface Chemistry of the Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China., Zhang Q, Liu A, Li X, Zhou H, Liu Y, Yan B |
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| Jazyk: | angličtina |
| Zdroj: | Journal of the American Chemical Society [J Am Chem Soc] 2011 May 11; Vol. 133 (18), pp. 6886-9. Date of Electronic Publication: 2011 Apr 15. |
| DOI: | 10.1021/ja111137n |
| Abstrakt: | We report a generic approach for identification of target proteins of therapeutic molecules using nanoprobes. Nanoprobes verify the integrity of nanoparticle-bound ligands in live cells and pull down target proteins from the cellular proteome, providing very important information on drug targets and mechanisms of action. As an example, target proteins as α-tubulin and HSP 90 have been identified and validated. (© 2011 American Chemical Society) |
| Databáze: | MEDLINE |
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